show Abstracthide AbstractAfrican catfish (Clarias gariepinus) is highly sought after for its meat quality and taste. It is extensively cultured worldwide in many countries in Africa, Europa, and Asia. A high-quality reference genome sequence with annotation of protein-coding genes is crucial for understanding important biological traits such as immune responses to various infectious agents, oxygen metabolism, physiological processes, and genetic breeding programs. This haplotype-resolve T2T genome assembly will provide resources to study this commercially valuable fish further.